Journal: Journal of ovarian research

Article Title: LncRNA NEAT1 participates in diminished ovarian reserve by affecting granulosa cell apoptosis and estradiol synthesis via the miR-204-5p/ESR1 axis.

doi: 10.1186/s13048-025-01683-6

Figure Lengend Snippet: Fig. 7 Silencing of ESR1 reversed the inhibitor-miR-204-5p-mediated biological effects on KGN cells. (a–b) KGN cell viability and proliferation were as sessed using CCK-8 and EdU assays. (c–d) Flow cytometric analysis of KGN cell apoptosis and cell cycle. (e) RT-qPCR was performed to detect the expres sion levels of key genes involved in steroid hormone synthesis. (f) Western blotting was performed to detect the expression levels of proteins related to steroid hormone synthesis and the MAPK signaling pathway. (g) ELISA was used to detect E2 levels. ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: Subsequently, a Human Estrogen E ELISA Kit (Cusabio, Wuhan, China) was used according to the manufacturer’s instructions, and the optical density was measured at 450 nm using an enzyme marker.

Techniques: CCK-8 Assay, Quantitative RT-PCR, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Journal of ovarian research

Article Title: LncRNA NEAT1 participates in diminished ovarian reserve by affecting granulosa cell apoptosis and estradiol synthesis via the miR-204-5p/ESR1 axis.

doi: 10.1186/s13048-025-01683-6

Figure Lengend Snippet: Fig. 8 NEAT1 regulates steroid hormone biosynthesis and the MAPK signaling pathway in KGN cells via the miR-204-5p/ESR1 axis. (a-b) RT-qPCR and western blotting were performed to evaluate the effect of NEAT1 on ESR1 mRNA and protein expression levels, respectively. (c) RT-qPCR was performed to analyze the expression of key genes involved in steroid hormone synthesis. (d) ELISA was performed to determine E2 levels. (e) western blotting was performed to determine the levels of proteins related to steroid hormone synthesis and MAPK signaling pathway. (f-g) RT-qPCR and western blotting were used to assess the expression levels of steroid synthase and key molecules of the MAPK pathway in human GCs, respectively. ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: Subsequently, a Human Estrogen E ELISA Kit (Cusabio, Wuhan, China) was used according to the manufacturer’s instructions, and the optical density was measured at 450 nm using an enzyme marker.

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay

Journal: International journal of molecular sciences

Article Title: Effects of Acrylamide on Mouse Implantation and Decidualization.

doi: 10.3390/ijms26094129

Figure Lengend Snippet: Figure 2. The concentrations of uterine E2 and P4 on days 4, 5, and 8 of pregnancy in mice treated with 0, 20 mg ACR/kg/d (20 mg ACR) and 30 mg ACR/kg/d (30 mg ACR) for 3 months, respectively. (a) The concentrations of uterine E2 on days 4, 5 and 8 of pregnancy. (b) The concentrations of uterine P4 on days 4, 5, and 8 of pregnancy. ns: no significant.

Article Snippet: Mouse E2 (CSB-E07280m, Cusabio, Wuhan, China) and P4 (E-OSEL-M0006, Elabscience, Wuhan, China) ELISA kits were used to analyze the concentration of E2 and P4 in uterine su- Int.

Techniques: